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CME Credit Offered
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Poster Session 2

(658) Differential expression of placental CD300 receptors in preeclampsia (PE) and preterm birth (PTB)

Thursday, January 30, 2025
4:00 PM – 6:00 PM  

    Submitting Author and Presenting Author(s)

  • William E. Ackerman, IV, MD (he/him/his)

    Research Associate Professor
    University of Illinois at Chicago, College of Medicine
    Chicago, Illinois, United States

    • Coauthor(s)

  • MT

    Meera M. Thakkar, MD, MPH

    Fellow
    University of Illinois at Chicago, College of Medicine
    Chicago, Illinois, United States

  • GZ

    Guomao Zhao, BS

    Laboratory Manager, Perinatal Research
    University of Illinois at Chicago, College of Medicine
    Chicago, Illinois, United States

  • ZS

    Zoe B. Strong, BA

    University of Illinois at Chicago, College of Medicine
    Chicago, Illinois, United States

  • EF

    Elizabeth Feoktistov

    University of Illinois at Chicago
    Chicago, Illinois, United States

  • ES

    Ekaterina Snegovskikh

    University of Illinois at Chicago
    Chicago, Illinois, United States

  • Catalin S. Buhimschi, MBA, MD

    Professor and Division Director Maternal Fetal Medicine
    University of Illinois at Chicago, College of Medicine
    Chicago, Illinois, United States

  • IB

    Irina A. Buhimschi, MD, MSc

    Professor and Director, Perinatal Research
    University of Illinois at Chicago, College of Medicine
    Chicago, Illinois, United States

Objective: Through their binding to lipids and other ligands, the 7 member CD300 family of receptors is poised to have a significant role in biological processes with an inflammatory component. Their roles in major inflammatory obstetrical syndromes such as PE and PTB +/- Triple I have not been fully elucidated. We performed a targeted computational analysis of existing placental datasets with transcriptional profiling to understand the expression and distribution of placental CD300 receptors in these pathological contexts.

Study Design:

We interrogated bulk and single-cell (sc) placental RNA sequencing (RNAseq) datasets (GEO: GSE73714, GSE203507, GSE114691, GSE173193; Figshare: 23264102) for CD300 expression in PE (n=65) and PTB (NO-Triple I n=26; YES-Triple I, n=5). This analysis was complemented by RNAseq in a new set of 8 fetal membrane (FM) samples as follows: YES-Triple I (n=4, GA 28±3w), NO-Triple I (n=4, GA 32±1w). Selected targets were cross validated by qPCR.

Results: Across placental RNAseq datasets, all 7 CD300 mRNAs could be detected, with CD300LG predominating in the villous placenta and CD300A and -E most abundant in FM. By scRNAseq, CD300LB, -E, and -LF displayed myeloid-limited expression, CD300A was expressed in myeloid and lymphoid cells, CD300LG was abundant in vascular endothelial cells, and CD300LC and -D expression fell below detection limits. Villous CD300LG was significantly more abundant in PE than non-PE samples (FDR < 0.05). In Triple I, CD300A, -LB, -LD, -E, -LF, and -LG were elevated (FDR < 0.05) in FM but not villous placenta by RNAseq, with CD300LF being most significant. Increased expression of placental CD300LG in PE and FM CD300LF in Triple I were confirmed by qPCR (p < 0.05).

Conclusion: The CD300 family of receptors is present in the human placenta. In pregnancy conditions with strong inflammatory components the signaling pathways emanating from this family of receptors are likely diverse based on disease and their location.